摘要
目的：考察将转化生长因子β（ＴＧＦ－β）、胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）、碱性成纤维细胞生长因子（ｂＦＧＦ）及血小板衍生性生长因子（ＰＤＧＦ）４种生长因子两两交互应用对人胚成骨样细胞增殖与分化的影响。方法：在不同的实验间期检测成骨样细胞３Ｈ－胸腺嘧啶脱氧核苷、３Ｈ－脯氨酸的掺入量和碱性磷酸酶的含量。结果：ＰＤＧＦ、ｂＦＧＦ、ＩＧＦ－Ⅱ和ＴＧＦ－β４种生长因子交互联合应用，均对促进体外培养的人胚成骨样细胞的增殖和分化功能具有协同作用。结论：具有协同作用的生长因子联合应用，可提高其促进骨形成的生物效应。
Objective:To examine the effects of platelet derived growth factor (PDGF), basic fibroblast growth factor (bFGF), insulin like growth factor (IGF II)and type β transforming growth factor(TGF β) which were believed to have biological effects on bone cells on human osteoblast like cells.Methods: 3H TdR, 3H Proline and alkaline phosphatase of the osteoblast like cells were tested to study the combinating effects of different growth factors on the proliferation and differentiation of osteoblast like cells.Results: The combination of different growth factors had promotive effect and synergic interact on proliferation and differentiation of osteoblast like cells.Conclusion: The phenomena indicate that the combination of two growth factors will enhance the biological effects of bone formation.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1999年第1期78-81,共4页
West China Journal of Stomatology
基金
国家教委博士点基金
CMB基金
关键词
生长因子
成骨细胞
细胞增殖
细胞分化
growth factor\ \ human osteoblast like cell\ \ proliferation and differentiation
