摘要
目的 应用双哌达莫 (DPM)、腺苷 (ADO)与 ADO拮抗剂茶碱 (TH)治疗博莱霉素 (BL E)肺纤维化小鼠 ,观察肺、脾等病理变化 ,探讨肺纤维化发病机理。方法 实验第 1天 10 0只小鼠经气管注入 BL E8.5 mg· kg- 1 ,随机分 BL E、 DPM、 ADO和 TH四组 ,第 2天起分别给予 NS (10 0μl· d- 1 )、 DPM、 ADO和 TH ,剂量均为 5 0 mg· kg- 1 · d- 1 ,共 7天 ,第 4～ 30天内处死动物 ,组织化学法观察肺内纤维变化与脾、胸腺、骨髓病理学改变。结果 BL E组和 TH组第 4～ 6天脾、胸腺髓质与骨髓中许多基质细胞坏死导致组织严重疏松。BL E组第 2 0天后脾与胸腺逐渐萎缩 ,肺与脾内网状纤维大量增加。DPM组与 ADO组第 4～ 6天脾、骨髓坏死基质细胞较少 ,第 30天肺未发生纤维化 ,淋巴器官未萎缩。结论 BL E能致脾、骨髓等基质细胞大量坏死与严重组织疏松。内、外源性 ADO能轻度减少基质细胞坏死 ,促进淋巴组织与造血组织增生 ,抑制 BL E诱导的肺纤维化与淋巴器官萎缩。
? Objective Pathologic changes of lung, spleen et al were observed and the machanism of lung fibrosis was explored by using dipyridamole (DPM), adenosine (ADO) and ADO receptor antagonist theophylline (TH) to treat lung fibrosis of mice induced by bleomycin (BLE). Methods 100 mice were injected 7mg/Kg of BLE intratracheally, divided at random into control, DPM, ADO and TH groups, perfused with 100μl·d -1 of NS and 50mg·Kg -1 of DPM, ADO and TH respectively from 2nd to 8th day, and executed from 4th^30th day. The lung fibers and pathologic changes of spleen, thymus and bone marrow were observed with histochemical methods. Results In the BLE and TH groups, form 4th to 6th day, many stromal cells of spleen, thymus medulla and bone marrow recvosed, resulting in serious loose tissue. After 20th day, thymus and spleen became atrophied, and the reticular fibers of lung and spleen increased markedly in the BLE group. In the DPM and ADO groups, however, there were a few live stromal cells in the spleen and bone marrow from 4th to 6th day, no atroply in the lymphoid organs and no lung fibrosis at 30th day. Conclusion BLE could cause serious stromal cell recrosis spleen, bone marrow et al. Endogenous and exopenous ADO can lightly reducd stromal cell necrosis, promote the cell proliferation of spleenic lymphoid and hemoopoietic tissue, and prevent BLE-induced pulmonary fibrosis and lymphoid organ atrophy. 〔
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2002年第1期89-91,共3页
Chinese Journal of Histochemistry and Cytochemistry
