摘要
【目的】优化罗望子壳总黄酮的提取工艺条件,并研究其对羟基自由基(·OH)的清除活性,为罗望子的药用开发提供科学参考。【方法】以罗望子壳为材料、乙醇为提取剂,通过单因素试验和正交试验,考察乙醇体积分数、料液比(g∶mL)、浸提温度、浸提时间对罗望子壳总黄酮提取率的影响,以优化罗望子壳总黄酮提取工艺条件;并以二丁基羟基甲苯(BHT)为对照,初步探究罗望子壳总黄酮提取液对·OH的清除活性。【结果】影响罗望子壳总黄酮提取效果的主要因素是浸提温度,其次是料液比和乙醇体积分数,浸提时间对罗望子壳总黄酮提取效果的影响相对较小;罗望子壳总黄酮最佳提取工艺条件为:乙醇体积分数75%,料液比1∶40,浸提温度80℃,浸提时间2.0 h,在此条件下罗望子壳总黄酮提取率为2.700%。罗望子壳总黄酮提取液对·OH清除活性高于相同质量浓度的BHT。【结论】乙醇浸提法是提取罗望子壳总黄酮的有效办法,且提取获得的总黄酮对·OH具有较强的清除能力。
[Objective]Contrastion of the total flavonoids content from different parts of tamarind and study on the scavenging effect on hydroxyl radicals provide a preparation for potential application.[Method] In this study,flavonoids from tamarind shell were extracted by using ethanol.The preferred extraction conditions were determined by single factor experiment and orthogonal experiment.[Result]The experiment reveals that：ethanol concentration of 75％,solid-liquid ratio of 1∶40（mg∶mL）,temperature was 85 ℃ and extraction time was 2.0 h were the preferred extraction conditions.The extraction ratio of flavonoids reached up to 2.700％ by using spectrophotometry.Antioxidant effects of flavonoids and BHT were measured to remove hydroxyl radicals by comparative experiments.Antioxidant experiments showed that flavonoids from tamarind shell have a strong scavenging effeet on hydroxyl radicals.[Conclusion]Extraction of total flavonoids by using ethanol was proved to be an effective technique,total flavonoids extracts obtained from tamarind shell has a strong scavenging effect on hydroxyl radicals
出处
《南方农业学报》
CAS
CSCD
北大核心
2014年第5期844-849,共6页
Journal of Southern Agriculture
基金
云南省应用基础研究计划项目(2012FD050)
楚雄师范学院科学研究基金项目(11YJGG01)
关键词
罗望子壳
总黄酮
提取率
清除活性
羟基自由基(·OH)
tamarind shell
flavonoids
extraction ratio
scavenging effect
hydroxyl radicals
orthogonal experiment
