摘要
目的 探讨胸水中淋巴细胞微核率 (PFL MNF)对良、恶性胸腔积液鉴别诊断的意义 .方法 对良、恶性胸腔积液患者及健康人各 2 0例采用细胞分裂阻滞微核 (CB- MN)法检测外周血淋巴细胞微核率 (PBL MNF)及用丝裂霉素 C(MMC)诱发 PBL MNF,直接用光镜观察 PFL MNF.结果 PFL MNF与 PBL MNF有良好的相关性 (r=0 .935 ,P<0 .0 1) .恶性胸腔积液患者自发 PBL MNF及诱发 PBL MNF均显著高于对照组和良性胸腔积液组 (P<0 .0 1) .良性胸腔积液组 PBL MNF与对照组无统计差异 (P>0 .0 5 ) .恶性胸腔积液组 PFL MNF较良性胸腔积液组明显升高 (P<0 .0 1) .结论 PFL MNF检测方法简单、快速、实用 ,并可以代替PBL MNF检测 ,PFL MNF检测可成为良。
AIM To investigate the significance of pleural fluid lymphocyte micronuclei frequency (PFLMNF) for diagnosis of malignant pleural effusion and benign pleural effusion. METHODS The modified cytokinesis-blocked micronucleus (CB-MN) technique was used to determine peripheral blood lymphocyte micronuclei frequency (PBLMNF) and mitomycin C (MMC)-induced PBLMNF in 20 cases withbenign pleural effusion, 20 cases with malignant pleural effusion and 20 health controls, while PFLMNF was investigated in the 40 patients at the same time. RESULTS There was a better correlation between PFLMNF and PBLMNF (r= 0.935, P<0.01). Spontaneous PBLMNF and MMC-induced PBLMNF of the malignant effusion group were much higher than that of the benign effusion group or the control group (P<0.01). However, PBLMNFs in the latter two groups had no statistic difference (P>0.05). PFMNF in the malignant effusion group were significantly higher than that in the benign group (P<0.01). CONCLUSION PFLMNF test, which is a simple, fast, practical method,can be used to replace the PBLMNF test. It is a practical diagnostic method for malignant pleural effusion and benign pleural effusion.
出处
《第四军医大学学报》
北大核心
2002年第6期549-551,共3页
Journal of the Fourth Military Medical University
