胸水脱落细胞DNA含量及VEGF,p53和CEA表达在良恶性胸腔积液鉴别中的意义被引量：8

Clinical significance of DNA content and VEGF, p53 and CEA expressions in the differential diagnosis of malignant and benign pleural effusions

暂未订购

导出

摘要目的探讨检测胸水细胞的DNA倍体以及VEGF、p53、CEA表达鉴别良恶性胸腔积液的价值。方法73例胸腔积液患者,良性组13例,恶性组60例。图像细胞光度技术(image cytometry,ICM)进行DNA含量检测,免疫组化Envision法检测VEGF、p53、CEA。结果良性胸水DNA异倍体率仅占23.1%。恶性胸水的异倍体率占77.8%,两者有统计学差异(P=0.001)。良性胸腔积液患者VEGF、p53、CEA表达率分别为12.5%、0、0。恶性胸腔积液VEGF、p53、CEA表达率分别为17.5%,17.5%和68.4%,与良恶性胸腔积液中CEA的表达相比有统计学差异(P<0.001),VEGF和p53的表达无显著差异(P=0.722,P=0.198)。经DNA倍体联合VEGF、p53、CEA检测,敏感率可达90.4%,特异度87.5%,符合率90%。结论ICM检测胸水细胞的DNA倍体联合VEGF、p53、CEA表达值得进一步探讨,有可能成为鉴别良恶性胸腔积液的又一方法。 Objective： To investigate whether nuclear DNA content and vascular endothelial growth factor （VEGF）, p53 and careinoembryonic antigen （CEA） expressions had a potential value in differential diagnosis of malignant and benign pleural effusions. Methods： Seventy-three patients with unilateral pleural effusion were divided into 2 groups： benign group （n = 13 ） and malignant group （n = 60）. The DNA content was measured by the image cytometry （ICM） and the level of VEGF, p53 and CEA expressions were detected by immunohistochemical Envision method. Results： The rates of aneuploid in benign and malignant pleural fluids were 23.1% and 77.8%, respectively. The difference between the benign and malignant pleural effusions was significant （ P 〈 0.001 ）. Immunohistochemical staining showed that the positive rates of the VEGF, p53, and CEA in benign pleural fluid were 12.5%, 0, and 0; in malignant pleural fluid were 17.5%, 17.5%, and 68.4%, respectively. The difference in CEA between the benign and malignant pleural effusion was significant （P 〈0.001 ）. The expression of VEGF and p53 had no statistically difference between the benign and malignant pleural effusions （P =0.722, P =0. 198）. Combined detection of DNA content, VEGF, p53, and CEA achieved 90.4% sensitivity, 87.5% specificity, and 90% consistent rate in diagnosis of malignant pleural effusion. Conclusion： Combined detection of DNA content, VEGF, p53, and CEA is worthy of further investigation. It has the potential to become an effective method in differential diagnosis of malignant and benign pleural effusions.

作者周箴陈岗宣学金张俭

机构地区上海市肺部肿瘤临床医学中心上海交通大学附属胸科医院病理科

出处《肿瘤》 CAS CSCD 北大核心 2007年第2期155-157,161,共4页 Tumor

关键词肺肿瘤胸腔积液恶性 DNA 基因表达免疫组织化学诊断鉴别 Lung neoplasms Pleural effusion, malignant DNA Gene expression lmmunohistochemistry Diagnosis, differential

分类号 R734.2 [医药卫生—肿瘤] R730.43 [医药卫生—肿瘤]

引文网络
相关文献

参考文献8

1许良中.实用肿瘤病理方法学[M].1版.上海:上海医科大学出版社,1996.
2吴莹星,漆秀梅,魏炜.血管内皮生长因子在卵巢上皮性癌中的表达[J].癌症,2000,19(6):565-568. 被引量：12
3沈策,叶曜芩,罗文侗,孔宪涛.用流式细胞仪对胸水细胞的DNA含量和细胞周期的研究[J].中华结核和呼吸杂志,1994,17(2):90-92. 被引量：15
4COHEN C.Image cytometric analysis in pathology[J].Hum Pathol,1996,27(5):482-493.
5杨健,周宜开.全自动图象细胞仪在肺癌痰细胞学诊断中的应用1.DNA异倍体作为标志物诊断肺癌[J].广东药学院学报,2004,20(5):549-551. 被引量：8
6THICKETT D,ARMSTRONG L,MILLAR A B.Vascular endothelial growth factor(VEGF)in inflammatory and malignant pleural effusions[J].Thorax,1999,54(8),707-710.
7KAYA A,POYRAZ B,CELIK G,et al.Vascular endothelial growth factor in benign and malignant pleural effusions[J].Arch Bronconeumol,2005,41(7):376-379.
8张贺龙,王文亮,闵婕.应用PCR-SSCP方法检测胸水细胞中p53基因突变鉴别良恶性胸水[J].第四军医大学学报,2000,21(7):869-871. 被引量：10

二级参考文献17

1白莉,孙燕,李申德.肺癌组织抑癌基因P53异常分析[J].癌变·畸变·突变,1996,8(6):321-326. 被引量：7
2范肇宏，上海医学，1987年，10卷，409页
3朱允中，中华结核和呼吸杂志，1984年，4卷，216页
4Jiang W，Oncogene，1989年，4卷，6期，923页
5Kim K J，Nature，1993年，362卷，841页
6Peter W, Payne RT, Thomas JS, et al. Sputum by Quantitative Microscopy: A Reexamination of a Portion of the National Cancer Institute Cooperative Early Lung Cancer Study [ J ]. Mayo Clinic proceedings, 1997,72 ( 8 ): 697.
7Gagneten CB, Geller GE, Saenz MC. Diagnosis of bronchogenic carcimoma through the cytologic examination of sputum with special reference to tumour typing [ J ]. Acta Cytol,1970,20:530.
8Johnston WW. Cytologic diagnosis of lung cancer: principle and problems[J]. Pathol Res Pract,1986,181: 1.
9Marek W, Krampe S. Automatisierte quantitative image-Zytometrie bronchialer sptilfltissigkeiten bei Verdacht auf broncho-pulmonale Tumoren: Vergleich mit Zytologie, Histologie und klinischer Diagnose [ J ]. Pneumologie, 1999,53: 583.
10Mareke W, Lang KN. Can Semi-automated image cytometry on induced sputum become a screening tool for lung cancer?[J]. European Respiratory Journal ,2001,18:942.