摘要
通过对检定菌的敏感性，检定培养基、展开剂和发酵液样品等影响因素的考察，建立了一种检测发酵液中西索米星浓度的方法。采用短小芽孢杆菌为生物显影检定菌，双层琼脂扩散法于37℃培养16～18h，控制检定培养基的PH为7．5～8．0，发酵液样品pH为4．5～7，0，展开剂为15％的KH2PO4，点样量为10μl，薄板和菌层接触时间为20min等检定条件，能用于发酵液中西索米星效价的直接测定，其线性回归方程为 Y＝0．0115X＋1．747，r=0．9877。在相同条件下，对536μg/ml的西索米星标准液连续测定6次，测定结果为（531．0 ±12．7）μg/ml，RSD=3．28％，该法具有较高的实验重复性和准确性，分析稳定性基本符合要求。
ABSTRACT: Based on the optimization of determinant factors such as bioassay medium, plate developingsolution, sampling paper, of which were affecting the accuracy of the potency assay of sisomicin in fermentation broth, adirect transfer blotting method, using methylene blue as staining indicatior to measure the inhibition zone was proposed.The linear regression equation was: Y =0.0115±1. 747; r = 0. 9877. In a comparison experiments with a knowbioassay potency of sisomicin 536μg/ml, the results in 6 sucessively determinations were (531. 0 ± 12. 7 ) μg/ml, RSD= 3. 28%.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2002年第4期224-226,236,共3页
Chinese Journal of Antibiotics
基金
福建省教委基金资助项目(No.K20011)
关键词
西索米星
薄层层析
生物显影法
发酵液
抗生素
KEYWORDS: Sisomicin
TLC combined with autobiography assay
Fermentation broth
