摘要
用甲基硝基亚硝基胍(MNNG)复制Wistar 大鼠胃癌模型,68周后,在实验组12只大鼠中,6只患腺胃腺癌(诱发率为50%),其中1只同时伴发前胃鳞癌。对照组未出现自发癌.大鼠在处死前1h 曾腹腔内注射~3H—TdR3.7μq/g,切片经放射自显影术处理,HE 染色,结果:腺胃癌6例均为高分化或低分化腺癌,累及肌层及浆膜。除例4外,标记指效为12.4～16,明显高于对照组4～6,标记细胞弥漫分布于癌灶内;非癌腺胃标记指数为6.2～10.4,均高于对照组;增殖区也向表面或深部扩展及聚集.提示:~3H—TdR 掺入后细胞标记指数和增殖区改变,为早期发现癌前病变和预测癌的复发和预后可能有帮助.
Rat gastric carcinoma was induced by MNNG.After 68 weeks of experiment,adenocarcino-ma of glandular stomach was produced in 6 of 12 experimented male Winstar rats,but no such tu-mor was observed in the control group of Glandular stomach cancers were well differentiated orpoor differentiated adenocarcinomas.They invaded the muscle layer and serous membranethrough the muscularis mucosa.One hour befor being killed,the rats received an intraperiponeal in-jection of ~3H—TdR at a dose level of 3. 7μBq/g body weight.Slides were covered with emulsion,after 4 weeks exposure,they were developed and stained with hematoxylin and eosin.The resultsare as fellows:The number of labelling—index per pit of the normal appearing MNNG—treatedmucosa was 6.2～10. 4,whereas it was higher than that in the controls(4～6).When comparedwith that in the controls,the position of labelled cells in carcinogen～treated mucosa extended tothe surface epithelium significantly and downward to the area closer to the muscularis mucosa.The LI in gastric cancers(12.4～16)was significantly higher than that in the controls,except rat4.The position of labelled cells in adenocarcinoma diffused in the area of gastric cancer lesion.The above result,suggests that cell kinetic changs may be helpful for the early diagnosis of precan-cerous lesion and prognostic evaluation of the patients with gastric carcinoma.
出处
《苏州医学院学报》
1991年第2期100-102,169,共3页
Acta Academiae Medicinae Suzhou
关键词
胃肿瘤
细胞动力学
gastric carcinoma
precancerous lesion
autoradiography
MNNG
