摘要
以Percoll作为分离介质,采用60%SIP、50%SIP2种浓度,经2次离心,成功地分离了12名健康供血者周周血的单核、淋巴和粒细胞,纯度分别为83%、85%和98%;得率分别为48%、49%和47%。常规涂片、瑞氏染色后形态学观察示细胞形态完整,台盼蓝拒染实验示细胞活力良好(拒染率>95%).
The authors report a discontinuous density gradient centrifugation technique for separatingthe human peripherial white blood cells into monocytes,lymphocytes,and granulocytes by meansof percoll as a purifying medium.The consecutive centrifugation was performed in SIP at a concentration of 60% and 50% for 2 times.The data indicate that the purity and recovery rate are83%,85% and 98%,and 48%,49% and 47% respectively.The cell smear shows that cellsare intact(Wright staining).The Trypane Blue exclusion test demonstrates a good cell viabilitywith an exclusion rate of more than 95%.
出处
《苏州医学院学报》
1991年第1期50-52,85-86,共3页
Acta Academiae Medicinae Suzhou
关键词
血细胞分离
PERCOLL
密度梯度分离
percoll
ficoll-hypaque
discontinuous density gradient centrifugation
blood cell separation
