摘要
本文用Percoll配成的不连续密度梯度(Percoll法)对C57BL、C_(3H)和CBA品系小鼠的脾细胞进行了分离,并用不同密度梯度层分离所得的细胞对~3H-TdR或^(51)Cr标记的YAC-1靶细胞进行杀伤功能检测.发现以50～60％、60～70％二个密度梯度层之间的细胞杀伤活性最高,poly I:C可以增强这种活性.并且在同一效靶比例下,这种效应细胞对YAC-1靶细胞的杀伤活性均高于用Ficoll-Isopague分离的脾细胞(Ficoll法)及常规制备的脾细胞(常规法),前者与后二者所获数据间呈显著性差异(p<0.01)。
Spleen cells of C57BL, C3H and CBA syngeneic mouse were separated by Ficoll?Isopaque gradient centrifugation and then the cells obtained were suspended in growth medium. The mononuclear cells in growth medium were further fractionated into 5 fractions by Percoll discontinuous density gradient centrifugation. The cells in the fractions with density of 50～60% and 60～70% (low density cells) showed: highest cytotoxity against YAC-1 target cells. These low density cells mediating natural killer(NK) activity bore morpholoical characteristics of LGL; the cytotoxic effect of these cells could be potentiated by poly I:C. The cytotoxicity of low density fraction al cells against YAC-1 target cells was then compared to that of thefraction by Ficoll-Isopaque density gradient centrifugation as well as unfractionarted splenocytes. The results showed that the former cells had higher specific cytotoxicity than the latter two with statistical significance (p<0.01).
出处
《上海免疫学杂志》
CSCD
北大核心
1989年第5期286-289,共4页
Shanghai Journal of Immunology
基金
国家自然科学基金
