摘要
以粉菠萝[Aechmeafasciata(Lindl.)Baker]、八宝剑(VrieseapoelmaniiHort.)、虎斑姬凤梨[Cryptanthuszonatus(Vis.)Beer]、七彩羞凤梨[Neoregeliacarolinae(Beer)L.B.Sm.]等的幼株茎段为外植体,以MS+NAA0.5mgL-1+BA5mgL-1为诱导培养基,MS+BA1mgL-1+NAA0.1mgL-1 为增殖培养基,可获得较好增殖效果。用1/2MS+NAA0.5mgL-1生根效果较好,以椰糠:砂=1:1,或椰糠:泥炭土:蛭石=1:1:1为基质移苗的成活率均达95%以上。
Micropropagationwasstudiedof fourbromeliadplants,Aechmea fasciata,Vriesea poelmanii,Cryptanthus zonatus andNeoregelia carolinae,culturedby stemexplants.Inductionmediumof MS+NAA0.5mg L -1 +BA5mg L -1 ledto buddingafteroneor twoweeksof culture.MS+BA1mg L -1 +NAA0.1mg L -1 wastheoptimummediuminsubculture.Theoptimumconcentrationof NAAwas0.5mg L -1 in rooting.Plantletstransplantedon mediacontainedcoconut-bant:sand=1:1or coconut-bant:turf:vermiculite(1:1:1)hadbettersurvivalrateupto95%.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2002年第1期63-68,共6页
Journal of Tropical and Subtropical Botany
关键词
凤梨科
离体培养
繁殖
观赏凤梨
Bromeliaceae
In vitro culture
Micropropagation
