摘要
应用基因枪法对水稻两用系培矮-64S进行了转化。质粒载体pKC-3串联了三个抗虫基因,将其转化成熟胚诱导形成的愈伤组织,共获得33株转基因植株。分别对R_0代植株不同的基因进行PCR及Southern blot分析,并对R_1代植株进行了PCR分析。结果表明,三个抗虫基因均已整合到水稻基因组并获得稳定遗传。
Transformation of two-line genetic male sterile indica rice variety Pei' ai 64S was conducted by particle bombardment. Three insect-resisting genes ligased into plasmid vector pKC-3 was introduced into calli derived from mature embryo and a total of 33 transgenic plants had been obtained. The PCR and Southern blot analysis of each different gene in Rg plants and also PCR analysis of each different gene in RI plants showed the integration of three insect-resisting genes into the genome of transgenic plants which could stably pass down.
出处
《实验生物学报》
CSCD
北大核心
2002年第1期42-46,共5页
Acta Biologiae Experimentalis Sinica
基金
国家转基因植物研究与产业化专项
编号J99-B-012
