摘要
油菜菌核病(Sclerotinia sclerotiorun)是油菜的一种主要病害,世界各国均有发生,造成产量损失和品质下降。人工接种鉴定育种材料和育成新品种的抗病性是抗病育种工作的一个重要环节。为此,各国学者做了大量的研究,创造了一些人工接种方法。但一些方法受外界环境条件影响较大,有些方法费工费时,实际应用不便。本文对离体叶片接种法进行了研究。试验于1988年在加拿大马尼托巴大学进行。一、材料和方法 1.接种体。从油菜菌核病菌的一个生理小种中单孢分离、培养出菌核。在20℃恒温和黑暗条件下。
Mycelium of an isolate of ScIerotnia sclerotrum from rapeseed was grown on PDA. 1/2PDA+1/2WA.1/4PDA+3/4WA and WA media inoculated leaves removed from the third leaf of 6-week old plants of susceptible Canadian cultivar 'Westar'. The best medium for artificial inoculation was 1/4PDA+3/4WA oaccording to reaction and average lesion diameters of leaves to mycelium of S.sclerotiorurn.
Mycelium of S. sclerorum was grown on 1/4PDA+3/4WA inoculated Chinese lines 81007 81002 609 (B. napus) and Bai5 (B.campestis). Westar was used as check. Results showed average lesion diameters of thoselines declined from 26.87％ to 58.59％ compared with Westar. The preliminary study reported here indicated that inoculation of detached leaves from immature plants of rapeseed may be used for screening of breeding material and variety,
出处
《安徽农业科学》
CAS
1989年第3期56-58,共3页
Journal of Anhui Agricultural Sciences
