摘要
目的 研究显示人和兔皮瓣微血管构筑的实验方法。方法 利用内源性碱性磷酸酶活性 ,采用组织化学Gomori Takamatsu钙钴法和硝基四唑氮蓝 5 溴 4 氯 3 吲哚磷酸盐 (NBT BCIP)显色法 ,观察人和兔皮瓣的微血管构筑。结果 两种方法均能良好显示人皮瓣的真皮内和真皮下血管构筑 ,而兔皮瓣均呈阴性。结论 内源性碱性磷酸酶活性存在明显的种属差异。Gomori Takamatsu钙钴法和NBT BCIP显色法 ,是两种用于研究人皮瓣微血管构筑的实用可靠的方法 。
Objective\ To study effective methods of showing the microvascular architecture of human and rabbit skin flaps. Methods\ According to endogenous alkaline phosphatase activity, Gomori\|Takamatsu method and NBT/BCIP method were used to observe the microvascular architecture. Results\ Both methods had capability to demonstrate the microvascular architecture of the human skin flap, whereas the examining results of rabbits were negative. Conclusions\ Great difference of endogenous alkaline phosphatase activity exists in different animals. Gomori\|Takamatsu method and NBT/BCIP method are useful in study of microvascular architecture of human skin flaps.\;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2002年第2期72-73,T001,共3页
Chinese Journal of Plastic Surgery
关键词
皮瓣
微血管构筑
碱性磷酸酶
整形外科
Skin flap
\ Microvascular architecture
\ Alkaline phosphatase
