摘要
用ＭＴＴ快速测定法及流式细胞仪分析法分析了葛根提取物（ＰＲ）对靶细胞人肝癌ＳＭＭＣ７７２１细胞的抗癌药效，结果葛根提取物有比较强的抗癌活性，与丝裂霉素（ＭＭＣ）联合用药抗癌活性显著增强，流式细胞仪分析细胞分裂周期各时象ＤＮＡ变化显示，ＰＲ作用后Ｓ期细胞明显减少，增殖指数（ＰＩ）降低，并可诱导凋亡峰，提示葛根提取物具有一定抗癌活性。
Aim:To study antitumor effect of Puerariae Radix (PR) to liver neoplasm.Method:culturing human liver neoplasm cells SMMC7721 in vitro and dealing with each kind of drug to analyse tumor cell killing rate in vitro by MTT and to content alteration of each phase of cell circle by flow cytometry (FCM) assay.Result:PR has significant antitumor effect. combined with MMC,the antitumor activity is stronger than singledrug group.DNA content displayed that Sphase cell DNA content decrease significantly.Proliferation Index (PI) deline and introduced apoptosis peak(AP peak).
出处
《中药药理与临床》
CAS
CSCD
1997年第6期27-29,共3页
Pharmacology and Clinics of Chinese Materia Medica
