摘要
目的 用cDNA阵谱比较鼻咽癌组织及正常组织的基因表达谱 ,研究鼻咽癌组织内修复相关基因的表达差异。方法 AtlashumancancercDNAexpressionarray 774 2 1杂交后 ,用AtlasImage1.0 1a分析滤膜杂交结果 ,RT PCR反应验证滤膜杂交结果。结果 在 5 88个肿瘤相关基因中 ,共有134个基因表达上调 ,88个基因表达下调。在有DNA损伤应答、修复、重组相关基因的C区中 ,表达上调的基因有 2 1个 ,与修复相关的有 6个 ;表达下调的有 4 4个 ,与修复相关的则有 12个。结论DNA修复相关基因的改变可能在鼻咽癌病理生理过程中起一定作用。
Objective To compare gene expression gene profilie of nasopharyngeal c arcinoma(NPC) tissue with that of normal nasopharyngeal tissues by cDNA array a nd to discuss possible functions of DNA repair related genes in NPC tissue. Methods After hybridization of atlas human cancer cDNA expression array 7742 1 , atlas hybridization results were analyzed by Atlas Image 1.01a software package. Using RT PCR was used to confirm the results. Results Of 63 differentially expressed genes in quadrangle C including DNA damage respon se, repair & recombination related genes, 6 DNA repair related genes were up regulated, 12 were down regulated. Conclusion DNA repair related genes may be involved in patho physiological process of nas opharyngeal carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第2期114-117,共4页
Chinese Journal of Oncology
基金
国家自然科学基金重点资助项目 (3 973 0 2 0 0 )
中华医学会基金重点资助项目 (96 65 5 )
