摘要
目的 建立测定马蔺子素的极谱法。方法 单扫描示波极谱法。结果 在 8 0× 10 - 3 mol·L- 1 Na2 B4O7 1 6× 10 - 2 mol·L- 1 KH2 PO4(pH 7 7)支持电解质中 ,马蔺子素有一灵敏的极谱还原波 ,其峰电位Ep =- 1 2 3V(vsSCE) ,二阶导数峰电流ip″与马蔺子素浓度为 1 5× 10 - 7～ 5 2× 10 - 6mol·L- 1 呈良好线性关系 (γ =0 9992 ,n =9) ,检出限为 6 0× 10 - 8mol·L- 1 。 13次测量 2 0× 10 - 6mol·L- 1 马蔺子素还原波二阶导数峰峰电流 ,相对标准偏差RSD为0 87%。结论 该方法灵敏、简便、快速 ,可用于原料药及胶囊中马蔺子素含量的测定。
AIM To propose a polarographic method for the determination of irisquinone. METHODS A reduction wave of irisquinone was recorded by single sweep oscillopolarography. RESULTS In 8 0×10 -3 mol·L -1 Na 2B 4O 7-1 6×10 -2 mol·L -1 KH 2PO 4 (pH 7 7) supporting electrolyte, a reduction wave of irisquinone with peak potential -1 23 V ( vs SCE) achieved high sensitivity. The 2nd-order derivative peak current of the reduction wave was proportional to irisquinone concentration in the range of 1 5×10 -7 ～5 2×10 -6 mol·L -1 (γ=0 9992, n =9). The detection limit was 6 0×10 -8 mol·L -1 . Relative standard deviation (RSD) was 0 87% by performing 13 independent measurements on 2 0×10 -6 mol·L -1 irisquinone. CONCLUSION The proposed method was sensitive, simple, rapid, and can be applied to the determination of irisquinone in raw medicine and capsule.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第3期207-209,共3页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目 ( 2 9875 0 17)
