摘要
以氯仿∶甲醇∶NaH2 PO4缓冲溶液 (pH值 4 .8) 3∶2 .5∶1为溶剂系统 ,上相为固定相 ,下相为流动相 ,从红河青叶胆生物碱中分得 5个成分 ,其中 3个成分与碘化铋钾试剂反应显桔红色 ,还用硅胶薄层板验证了分离效果 ,并用光谱法证实了其中一个生物碱为龙胆碱 ,含量 30 %
Alkaloids from Swertia yunnanensis were isolated successfully by high speed countercurrent chromatography with the two phase solvent system CHCl 3 ∶CH 3 OH∶NaH 2 PO 4 (pH 4.8)3∶2.5∶1 to obtain 5 components,in which 3 components exhibited orange red color with bismuth potassium iodide reagent.Results were verified by TLC and five components were isolated pure.One was characterized as gentianine(30%)by spectroscopic analysis.
出处
《林产化学与工业》
EI
CAS
CSCD
2002年第1期74-76,共3页
Chemistry and Industry of Forest Products
基金
云南省教委基金资助项目 (984 2 0 6 2 )
