摘要
目的研究中药复方在缺血性脑损伤时对脑组织的保护作用。方法 75只Wistar大鼠随机分为 5组。第 1组为伪手术组 ,其余 4组剥离颈动脉后结扎 ,并切断颈总动脉。第 2组为造模组。术后第 2 0h起 ,第 3、4、5组分别灌喂中药复方水煎液Ⅰ、Ⅱ、Ⅲ号方各 1次。给药 3h后 ,第 2— 5组分别置于低氧环境 1h ,以后连续给药 7天 ,每天 1次。术后第 7天从右心房取血 ,处死动物 ,取全部右脑 ,分别测定脑组织和血清中的MDA、NOS、SOD及脑组织钙的含量 ,并观察海马CA1区锥体细胞的排列情况。结果Ⅰ、Ⅱ、Ⅲ号方均可降低脑组织和血清MDA的含量 ,并能降低脑组织钙的含量 (P <0 .0 5— 0 .0 0 1)。Ⅰ号方还可增加脑组织SOD的活性 ,Ⅱ、Ⅲ号方可降低脑组织NOS的活性。第 3、4、5组海马CA1区锥体细胞排列较整齐 ,坏死数目较造模组减少 ,其中第 4、5组更为明显。结论中药Ⅰ、Ⅱ。
ObjectiveTo study the protection of Chinese herbs on the ischemic brain of rats. Methods 75 Wistar rats were divided into 5 groups, Group 1 for false operation. For the other four groups, the common carotid artery was exposed then was ligatured and cut off, Group 2 for model. From the 20th hour after operation on,Group 3, 4, 5 were fed with complex prescription of Chinese medicine named Prescription 1, 2 and 3 once a day respectively. 3 hours after taking medicine, Groups 2-5 were put in the hypoxia environment for 1 hours, then taking the medicine for 7 days. On the 7th day after operation,the blood was taken from R. atria then the rats were killed and the whole right brains were cut off. Malonaldehyde (MDA), notric oxide synthetase (NOS) and superoxide dismutase (SOD) in the brain tissue and serum, and calcium in the brain were measured respectively. ResultsThe three prescriptions can decrease the quantity of MDA both in brain tissue and serum and the calcium in brain tissue( P<0.05-0.001). Prescription 1 can enhance the activity of SOD in brain tissue while the others can decrease the activity of NOS. The hippocampus cells show tidy, and the number of the necrotic cells decrease greatly among them, Groups 4 and 5 are better than Group 3.Conclusions Prescriptions 1-3 can protect the brain tissue of the rat form ischemic brain injury.
出处
《中国康复理论与实践》
CSCD
2002年第1期13-15,共3页
Chinese Journal of Rehabilitation Theory and Practice
关键词
中药
方剂
缺血性脑损伤
丙二醛
一氧化氮合成酶
超氧化物歧化酶
动物实验
脑损伤
Chinese herbs
complex prescription
ischemic brain injury
malonaldehyde (MDA)
notric oxide synthetase (NOS)
superoxide dismutase (SOD)
calcium
experiment
