摘要
目的 研究c met基因蛋白及增殖细胞核抗原 (PCNA)在幽门螺杆菌 (Hp)感染的胃粘膜病变演进中的表达及关系。方法 对 14 5例经病理证实的不同胃粘膜病变 ,采用免疫组化法检测c met和PCNA基因表达 ,Warthin starry法检测Hp感染。 结果 在浅表性胃炎 (CSG )、萎缩肠化生胃炎 (CAG +IM )、异型增生 (ATP)、早期胃癌和进展期胃癌中 ,CAG +IM、ATP、胃癌c met和PCNA两种基因均显著高于CSG(P <0 .0 5 )。胃粘膜增殖程度与c met和PCNA阳性表达强度显著相关 (P <0 .0 1)。c met和PCNA阳性表达与胃癌组织类型、浆膜浸润和淋巴结转移密切相关。c met LI和PCNA LI在胃癌中等级相关表达有极其显著的相关性 (P <0 .0 0 1)。c met阳性表达与肠型胃癌Hp感染有关。CAG +IM、ATP和GC组c met阳性表达中Hp感染者明显高于阴性者。结论 c met和PCNA基因表达与胃粘膜增殖和恶化有关。c met基因在Hp感染时其表达升高 ,是其恶化的主要参考指标。Hp感染和c
Objective To determine the relationship between the expression of c met oncogene and proliferating cell nuclear antigen (PCNA) in Helicobacter pylori(Hp) infectious gastric mucosal lesions.Methods Expression of c met and PCNA were investigated in 145 gastric mucosal lesions by using immunohistochemistry.Hp was examined by both of Warthin starry method and RUT.Results For chronic superficial gastritis(CSG),chronic atrophic gastritis with intestinal mataplasia (CAS+IM),Atplasia (ATP),early GC and advanced GC,the positive rates of c met and PCNA were higher in IM,ATP and GC than in CSG (P<0.05).Expressions of c met and PCNA increased with the increasing proliferation of gastric mucosa,they had a close relationship (P<0.01).Expressions of c met and PCNA were correlated significantly with histologic types,serosal invasion and lymph node metastasis.c met and PCNA have a close relationship (P<0.001).Expressions of c met in Hp positive group were higher than that in Hp negative in intestinal GC (P<0.05).Expressions of c met were correlated significantly with Hp positive in CAG+IM,ATP and GC.Conclusions The expression of c met may be associated with the proliferation and malignant transformation of gastric mucosa.Expression of c met may be a malignant transformation factor in Hp infection.Hp infection and expression of c met may be associated with in proliferation and malignant transformation of gastric mucosa.
出处
《临床内科杂志》
CAS
北大核心
2002年第2期114-116,共3页
Journal of Clinical Internal Medicine
