摘要
研究实验犬食管支架术后对局部粘膜血流量、PEG40 0 通透性以及电镜下局部粘膜形态的变化。选择成年健康犬均分为 2组 ,置入“Z”型食管支架 ,采用多谱勒血流仪检测局部粘膜血流量的变化。支架术后 2h选择第 1组实验犬进行食管持续恒压PEG40 0 灌流 ,通过气相色谱分析法检测组织中PEG40 0 的含量。第 2组实验犬于术后 2 4h同样方法进行气相色谱分析。电镜分析支架术后 2h及2 4h粘膜结构的变化。结果发现支架术后局部粘膜血流量较正常食管明显减少 ;术后 2h食管组织中PEG40 0 的含量较正常明显升高 ,术后 2 4h则进一步增高 ;电镜发现支架术后食管上皮细胞间连接及基底膜破坏 ,术后 2 4h较 2h变化更为明显。由此可见支架置入可导致食管局部粘膜血流量明显降低 ;支架术后上皮细胞间连接及粘膜结构破坏明显 ,对PEG40 0 通透性显著增高。
To observe the changes in mucosal blood flow and permeability after esophageal 'Z'stenting, 12 adult healthy dogs were divided into two groups, and an esophageal stent was placed in each dog. Mucosal blood flow was measured with Dopplar blood flowmeter. Two hours after stenting PEG400 was instilled into the esophagus continuously under a constant pressure for two hours in the first group. The amount of PEG400 in the stented tissue was measured with gas liquid chromatography.In the second group the same experiment was carried out 24 hours after stenting. The mucosal structure was examined with electron microscope after stenting. It was found that the mucosal blood flow decreased evidently, and the amount of PEG400 was much greater in tissue after being stented as compared with that in normal esophagus. Electron microscopy revealed that the number of desmosome of epithelium decreased, and it was disrupted significantly after stenting. The interval epithelial cells were enlarged, basement membrane was damaged. Stenting might lead to evident decrease of esophageal mucosal blood flow, which significantly impaired the junction of epithelial cells and mucosal structure, and the permeability for PEG400 was increased significantly. All these findings demonstrate that stenting might impair the esophageal mucosa.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第3期236-238,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题 (编号 39670 341 )
