摘要
目的观察逆行灌注浓缩红细胞对深低温停循环鼠脑的影响。方法将SD大鼠随机分成假手术组(Shamop组,n=12)、单纯深低温停循环组(DHCA组,n=12)和逆行灌注浓缩红细胞组(DHCArcp组,n=12),建立深低温停循环模型(18℃,90min)。模型成功后取脑作光镜和电镜分析并测量脑组织湿/干重比。结果光镜和电镜结果均证明DHCArcp组比单纯DHCA组脑组织损伤程度轻(P<0.05);两组脑组织的湿/干重比值没有明显差异(P>0.05)。结论逆行灌注氧合浓缩红细胞具有脑保护作用,并且不增加术后的脑水肿。
ObjectiveTo observe the effects of retrograde cerebral perfusion of oxygenated red blood cells on the rat brain after deep hypothermic circulation arrest.MethodsThirty-six SD rats were divided into three groups: sham operation group(sham op, n=12), deep hypothermic circulation arrest alone group(DHCA, n=12) and (retrograde) cerebral perfusion of oxygenated red blood cells group(DHCArcp, n=12). After set up of the model of DHCA(18 ,90 min) the brains were dissected and the pathological changes were examined by the light microscope and electron microscope, the wet/dry ratio of the brain was recorded at the same time.ResultsLight microscopy and electron microscopy revealed that the injury of brain in DHCArcp group was less than DHCA group, but the wet/dry ratio of the brain had significant difference between the groups.ConclusionRetrograde cerebral perfusion of oxygenated red blood cells does have protective effect and does not result in brain edema after DHCA.
出处
《上海第二医科大学学报》
CSCD
北大核心
2005年第7期690-693,共4页
Acta Universitatis Medicinalis Secondae Shanghai
基金
上海市卫生局基金(01460)资助项目.
关键词
深低温停循环
逆行灌注
脑
大鼠
<Keyword>deep hypothermia circulation arrest
retrograde cerebral perfusion
brain
rat
