摘要
目的研究人癌胚抗原重组痘苗病毒(rV-CEA)转染外周血树突状细胞(DC)后能否在体外诱导CEA特异性细胞毒性T淋巴细胞免疫。方法将rV-CEA转染外周血单核细胞来源的DC后用于激发同源的T细胞,检测其对T细胞的增殖作用以及对CEA分泌性肿瘤细胞的杀伤活性,并与未经rV-CEA转染的DC激发的T细胞进行比较。结果经rV-CEA转染的DC激活的T细胞对CEA分泌性肿瘤细胞具特异性杀伤作用。结论rV-CEA转染的DC可以诱导CEA特异性T细胞活性。
Objective To observewhetherdendriticcells(DCs)transfectedwithcarcinoembryonicantigen(CEA)-vaccinia recombinantvirus(rV-CEA)inducescytotoxicT lymphocyte-mediatedCEA-specificimmunityin vitro.Methods DCs derivedfromperipheralbloodmonocytesweretransfectedwithrV-CEAandthencoculturedwithautologousT cells.The proliferationof theinducedT cellsandtheircytotoxicactivityagainstCEA-secretingtumorcellswereassessedandcompared withthoseof T cellsinducedby DCswithoutrV-CEAtransfection.Results T cellsinducedby DCstransfectedwithrV-CEA showedspecificcytotoxicityagainstCEA-secretingtumorcells.Conclusion DCs transfectedwithrV-CEAcan elicit activationof CEA-specificT cells.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第3期256-258,共3页
Journal of First Military Medical University
