摘要
采用比色法、荧光和吸收光谱法研究了N -邻香草醛氨基葡萄糖希夫碱 (O -Vanillinwithgiucosamine,VG)的铜 (Ⅱ )、锌 (Ⅱ )、钴 (Ⅲ )配合物 (简称 :MVG)对超氧阴离子自由基和DNA的作用 结果表明 :CuVG ,ZnVG和CoVG能抑制超氧离子自由基的生成 ,抑制能力最强的是CuVG 此外 ,配合物CoVG可与DNA有效结合 ,结合常数 (Ko)值为 5 .2 6× 1 0 4 mol-1·L ,每 1 0 0个核苷酸的片段可提供结合位点数是 3
The effects of O-Vanillin with D-glucosamine(VG) S chiff base complexes(MVG) with Cu(Ⅱ)?Zn(Ⅱ)?Co(Ⅲ) on O^- 2~· and DNA were studiedThe results showed that CuVG,ZVG and CoVG can inhibit the sy nthesis of O^- 2~· markedlyThe inhibition ability best was CuVGIn th e meantime,complexes CoVG could combine with the salmon sperm DNAThe intrinsic association constant (Ko) for CoVG were estimated to be 5.26×10~4 M^-1 The number of binding sites per 100 nucleotide(n) was 38
出处
《三峡大学学报（自然科学版）》
CAS
2001年第5期477-480,共4页
Journal of China Three Gorges University：Natural Sciences
