摘要
采用随机扩增多态ＤＮＡ（ＲＡＰＤ）技术对淤泥湖和梁子湖的团头鲂进行了遗传分析．在所用２０个１０碱基随机引物中，除ＯＰＰ－１、ＯＰＰ－１３外，其余１８个引物都能对团头鲂扩增出稳定的ＲＡＰＤ谱带，其中ＯＰＰ－７、８、９、１２、１５、１７等６个引物在团头鲂不同个体间扩增出多态性片段．用这些引物对团头鲂进行遗传分析，结果表明，淤泥湖团头鲂个体间的遗传相似度在０．９５４３～０．９７１８之间，平均值为０．９６１７；梁子湖团头鲂个体间的遗传相似度在０．９５４１～０．９６２２之间，平均值为０．９５８９；淤泥湖和梁子湖团头鲂个体间的遗传相似度在０．９３９５～０．９６１４之间，平均值为０．９５４１．
Random Amplified polymorphic DNA(RAPD) was used to study the genetic diversity of bluntnose black bream from Yunihu and Liangzihu Lakes. Among 20 decamer primers studied, 18 primers gave stable results, while 6 primers produced polymorphic bands among different individuals of bluntnose black bream. Genetic similarity between individuals from Yunihu lake was 0.9617 with range from 0.954 3 - 0.9718; and genetic similarity between individuals from Liangzihu lake was 0.9589 with range from 0.9541-0.9622.
出处
《三峡大学学报（自然科学版）》
CAS
2001年第3期282-285,共4页
Journal of China Three Gorges University：Natural Sciences
基金
原湖北三峡学院青年科学研究项目(Y98027).
