摘要
根据鱼类外周血细胞都有核的特点， 采用从冷冻和低渗双重处理分离的细胞核提取基因组ＤＮＡ． 以此法获得的白鲢和鳙鱼的基因组ＤＮＡ为模板， 和Ｏｐｅｒｏｎ 公司生产的ＯＰＮ 和ＯＰＭ 两个组共４０ 个随机引物， 对这两种鱼进行了随机扩增多态ＤＮＡ （ＲＡＰＤ） 分析； 确定了对这两种鱼基因组相关区域可进行随机ＰＣＲ扩增的有效引物， 特别是哪些可产生种群内或群体的ＲＡＰＤ 遗传标记， 即可产生个体特异性和群体特异性ＲＡＰＤ带谱的引物． 讨论了ＲＡＰＤ遗传分子标记在鱼类遗传， 特别是遗传多样性研究， 和鱼类种质资源评估和管理中的应用前景问题．
Total 40 random primers of OPN and OPM groups made in Operon Co. were applied to the RAPD analysis of H.molitrix and A.nobilis . Besides establishment of species specific RAPD electrophoretogram, some kinds of primers which can produce molecular genetic markers of RAPD with individuality or population specificity of these fish were found. A modified method to extracting genomic DNA from peripheral blood cells of fish, and the application prospects of RAPD technique in genetic diversity and variation of fish, and in fishery management and the evaluation of fish resources were discussed.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第5期469-472,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
