摘要
用细胞融合法获得两株稳定分泌抗人血型A抗原单克隆抗体的杂交瘤细胞株(HA_3和HA_4)。抗体特异性很好,与“O”和“B”型红细胞无交叉凝集,千余份血型测定结果与标准血清一致;抗体为IgM类,耐热、耐冻融,可望代替标准血清供市售生产。
<ABSTRACT> Using cell fusion technique two hybridoma cell lines secreting specific monoclonal antibodies (McAbs)against human blood group A antigen(HA_3 andHA_4) were established. The specificity of these two McAbs is satisfactory.They didn′t agglutinate with type“O”or“B”of human blood red cells. More than a thousand human blood samples had been examined with these twoMcAbs. The results were well consistent with that of standard serum. Both HA_3 and HA_4 McAbs were of the class IgM, resistant to heat and freezingthawing. If be produced commercially, it is possible to replace the standardblood group typing serum.
关键词
细胞融合
杂交瘤细胞株
单克隆抗体
myeloma
cell fusion
hybridoma cell lines
monoclonal antibodies against human blood group A antigen
mice
