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Mip3β、血型A抗原模拟多肽/Fas融合基因双表达重组质粒的构建及鉴定 被引量：1

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摘要 目的:构建Mip3β、血型A抗原模拟多肽/Fas融合基因双表达重组质粒。方法:通过RT-PCR的方法从扁桃体中扩增的Mip3β基因片段,然后将基因工程合成的血型A抗原模拟多肽/Fas融合基因经双酶切后分别连接至pIRES质粒,测序验证碱基序列。结果:扩增出了Mip3β的全长CDNA,并成功构建Mip3β、血型A抗原模拟多肽/Fas融合基因双表达重组质粒,测序结果正确。结论:成功构建了Mip3β、血型A抗原模拟多肽/Fas融合基因双表达重组质粒,为进一步探讨血型抗原模拟多肽对肿瘤微环境的影响奠定分子学基础。
作者 邹建军 李许锋 岑东芝 孟辉 郭敏 张积仁
机构地区 南方医科大学附属珠江医院肿瘤中心
出处 《实用医学杂志》 CAS 北大核心 2011年第4期569-571,共3页 The Journal of Practical Medicine
关键词 质粒 血型A抗原 模拟多肽 Mip3β FAS
分类号 R392.1 [医药卫生—免疫学]
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参考文献10

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二级参考文献18

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实用医学杂志
2011年 第4期

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