摘要
用SLDT技术、细胞生长曲线、~3H-TdR掺入、细胞分裂指数和流式细胞光度术等方法在V_(79)细胞和WB肝细胞中观察到中药促癌物黄芫花提取物刺激细胞增殖的作用与对细胞GJIC抑制的作用有平行相关性。黄芫花提取物抑制V_(79)细胞的GJIC,同时刺激DNA合成,使细胞周期M期时相百分比升高,与TPA的效应相似,对黄芫花提取物促癌作用机理、GJIC抑制与细胞增殖周期的相关性进行分析讨论。
A Chinese herb, wikstroemia Chamaedaphen (WC) extract, recently has been shown to be a potential tumor promoting agent on uterine cervical carcinoma induced by HSV-2 or MCA in mice. To determine whether the tumor promoting effects of WC extract were mediated through inhibition of gap junctional intercellular communication (GJIC) with relation to cellular growth, experiments were conducted on Chinese: hamster V 79 cells and rat WB liver cells by utilization of SLDT method for GJIC detection and cell growth curve examination, ~3H-TdR incorporation, mitotic index (MI) and Flow Cytometry (FCM) methods. TPA was used for comparative purpose. WC extract inhibited GJIC and stimulated cell growth in a dose (2--200 μg/ml) and time (0—72 hr)-depcndent manner in both cell lines. Both WC extract and TPA treatments increased V 79 cell growth rate. The average cell doubling-time was deereased from 36.5 hr in control V79 cells to 28.2 hr in WC extract (10μg/ml) and 20.9hr in TPA (50ng/ml)trentment by the 3rd day. Stimulating effect of both drugs on DNA synthesis of V79 cells was demonstrated. The results of FCM and MI indicated theft the cell number of M-phase cells was increased after drug treatment. It is suggested that (1) tumor promoting effect of WC extract might be mediated through inhibition of GJIC; (2) inhibition of GJIC is closely correlated with increased cell growth rate and entry of cell division cycle.
出处
《实验生物学报》
CSCD
1991年第4期307-315,共9页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
关键词
黄芫花
提取物
细胞
增殖
生物学
WC extract. TPA. GJIC, Cellular growth. Tumor promotion.
