摘要
用透射电镜观察到MGC-803细胞的核仁是网织型的,在网眼内分布有电子密度低的纤维中心。MGC-803细胞经丁酸钠作用后,其核仁的类型发生了改变,多呈环型的,核仁的中央有一个大的纤维中心;纤维中心和银染颗粒的大小和数目明显减低;用图像分析仪测得核仁银染蛋白所占面积与核总面积的比值也明显降低。结果提示:丁酸钠可能通过抑制rRNA合成和rDNA转录活性调控MGC-803细胞的增殖。
The changes in the ultrastructure of fibrillar centres, morphological type of nucleolus, and the content of Ag-nucleolus protein of Sodium Butyrate treated MGC-803 cells have been studied. It was found that the number of fibrillar centres and Ag-staining granules, content of Ag-staining protein of nucleolus were decreased, and nucleolar morphological type was changed from reticulated to ring-shaped in NaBT treated MGC-803 cells.These results indicate that NaBT regulate the proliferation and differentiation of cells by inhibiting rRNA synthesis and rDNA transeriptional activity.
出处
《实验生物学报》
CSCD
1991年第4期317-323,共7页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
关键词
胃肿瘤
腺癌
细胞
丁酸钠
核仁
MGC-893 cells. Sodium Butyrate (NaBT) .Nucleolus. Fibrillar centre. Agstaining protein.
