摘要
作者曾利用Krens等(1982)的PEG法将NPT-Ⅱ丛因直接导入绿豆原生质体并获得稳定表达,但该法的转化率极低。为了建立绿豆原生质体有效的转化系统,我们将PEG法在大豆原生质体转化的基础上进一步改进,利用GUS基因为报告基因,对三个不同品种的绿豆叶肉原生质体进行直接转化。并对影响PEG转化的有关因素进行了研究。
The E. Coli beta-glucuronidase (GUS) gene was used in transient expression experiments with mesophyll protoplasts of Phaseolus aureus. Transient expression of GUS gene could be detected 44 hours after DNA incorporation into protoplasts by using a flurogenic substrate MUG. The results indicated that transformation efficiency was increased by increasing plasmid DNA concentration, carrier DNA was not beneficial when transforming with circular plasmid DNA, and PEG 40% (13.3% in final) was more suitable than PEG 24% (8% in final) when other affecting factors were identical.
出处
《实验生物学报》
CSCD
1991年第4期431-434,共4页
Acta Biologiae Experimentalis Sinica
关键词
绿豆
原生质体
外源基因
瞬间表达
Phaseolus aureus. Protoplast. Transient Expression.
