摘要
目的 :探讨‘危机血清’介导疟原虫配子体对蚊感染力的作用机制。方法 :通过间接免疫荧光实验和直接蚊血食实验 ,观察感染第 5天小鼠血清 (D5血清 )对配子体感染力的影响。利用ELISA和Griess反应实验 ,检测D5血清对感染小鼠血清中IFN γ、TNF α、IL 4和脾细胞培养上清中NO-2 产生的影响。同时 ,检测了疟原虫寄生红细胞的提取物 (PRBC提取物 )诱导NO合成的能力。结果 :感染第 5天小鼠血液中的配子体向动合子的发育完全发生阻断。D5血清注入感染第 3天的小鼠体内后未见立即抑制配子体在蚊体内的发育 ,但 4h后 ,可促进宿主体内IFN γ增多 ,提高NO的表达 ,并抑制配子体的感染力。并且 ,PRBC提取物诱导感染小鼠脾细胞NO的合成。结论
Objective:To clarify the mechanism of crisis serum' mediated gametocyte infectivity to the mosquito vector Methods:Observing the effects of mouse serum , which was obtained 5 days after P yoelii infection (D5 serum) on gametocyte infectivity by IFA and mosquito live feeds, and the production of IFN γ?TNF α?IL 4 and NO - 2 in the hosts in vivo and in vitro by ELISA and Griess reaction And to investigate the ability of malaria parasitized red blood cell extract (PRBC extract) to induce NO Results:The development of the gametocytes from mice 5 days postinfection into ookinetes were completely inhibited D5 serum was not immediate to inhibit gametocyte development, which was injected intravenously into the mice 3 days after P yoelii infection But 4 h later after injection D5 serum stimulated the increasing IFN γ and NO production and inhibited gametocyte infectivity Moreover, PRBC extract showed the ability to induce NO Conclusion:Infected host serum blocks transmission of P yoelii via a nitric oxide dependent mechanism
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第8期419-423,共5页
Chinese Journal of Immunology
关键词
约氏疟原虫
危机血清
一氧化氮
配子体感染力
Plasmodium yoelii(P.yoelii) Crisis serum Nitric oxide Infectivity of gametocyte
