摘要
目的 :研究IFN -γ和TNF对结核分枝杆菌感染小鼠的疗效及部分作用机制。方法 :小鼠感染结核分枝杆菌后第 5天给予IFN -γ和 /或TNF治疗 ,观察治疗后半数死亡时间、一定时间内的死亡率、脾内活菌数 ,检测Mφ产生NO水平 (用Griess试剂 )。结果 :联用IFN -γ和TNF组与单纯攻毒组比较明显延长感染小鼠半数死亡时间、降低感染小鼠 35天内的死亡率、显著减少感染小鼠脾内活菌数 ,Mφ产生NO水平明显增加。 结论 :IFN -γ和TNF联用对结核分枝杆菌感染小鼠产生保护效应 ,其机制可能与增强Mφ释放NO水平有关。
Objective To study the therapeutic effect and mechanism of IFN-γ combined with TNF on mycobacterium tuberculosis infected mice.Methods The mice were treated with IFN-γ and /or TNF 5 days postinfection challenged with H37RV by their tail veins. Time of fifty percent death (T50) and mortality rate within 35 days were investigated. The numbers of viable bacteria in spleen were counted. The level of nitric oxide (NO) in macrophage cultural supernatant was measured using Griess reagent.Results The combination of IFN-γ with TNF group prolonged T50, decreased mortality rate within 35 days, diminished the numbers of viable bacteria in spleen, enhanced the production of NO secreted by Mφ. There was significant difference compared with infection control group (P<0.01).Conclusion The combination of IFN-γwith TNF can enhance the host defense against the infection with H37RV.It may be related with increase of the level NO by Mφ.
出处
《郧阳医学院学报》
2001年第4期206-208,共3页
Journal of Yunyang Medical College
