摘要
目的研究 IFN- γ和 TNF对结核分枝杆菌感染小鼠的疗效及作用机制。方法小鼠感染结核分枝杆菌后第 5d给予 IFN-γ和 /或 TNF治疗 ,观察治疗后半数死亡时间、一定时间内的死亡率、脾脏内活菌数 ,检测脾细胞分泌 IFN-γ和 IL -4水平 (EL ISA法 )及 MΦ 产生 NO水平 (用 Griess试剂 )。结果与单纯攻毒组比较 ,联用 IFN- γ和 TNF组感染小鼠半数死亡时间明显延长、35 d内的死亡率从 10 0 %降低到 2 0 %、脾脏内活菌数显著减少 ,脾细胞 IFN- γ分泌水平明显升高 ,IL- 4分泌水平显著降低 ,MΦ产生 NO水平明显增加。结论 IFN -γ和 TNF联合诱导 MΦ产生 NO,促进 TH1 细胞反应 ,抑制 TH2 细胞反应 ,改变了 TH1 / TH2 平衡 。
ObjectiveTo study the therapeutic effect of IFN γ combined with TNF on mycobacterium tuberculosis infected mice and related mechanism Methods The mice were treated with IFN γ and/or TNF by intraperitonealy injection(ip)5 days postinfection challenged with H 37 R V by their tail veins Time of fifty percent death (T 50 ) and mortality rate within 35 days were investigated The numbers of viable bacteria in the spleen were counted The level of IFN γ and IL 4 in ConA induced spleen cell cultural supernatant were detected with ELISA method The level of nitric oxide(NO) in macrophage cultural supernatant was measured using Griess reagent Results The combination of IFN γ with TNF group prolonged T 50 , decreased mortality rate within 35 days, diminished the numbers of viable bacteria in the spleen, increased the level of IFN γ and reduced the level of IL 4 secreted by spleen cells,enhanced the production of NO secreted by MΦ There was significant difference compared with non treated infection group( P <0 01) Conclusions The combination of IFN γ with TNF induced NO production by MΦ,promoted development of T H1 cell response, inhibited T H2 cell response, modulated T H1 and T H2 cytokine balance, suggesting enhanced host defense against infection with H 37 R V.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第6期457-459,共3页
Immunological Journal
