摘要
目的 :探讨久泄 (慢性结肠炎 )脾胃湿热证与结肠镜像、炎症细胞和 P16、转化生长因子 - α(TGF- α)表达的关系。方法 :选取符合久泄脾胃湿热证患者 34例 ,脾胃气虚证 31例和正常对照组 11例进行结肠镜检查及炎性细胞和 P16、TGF- α测定。结果 :脾胃湿热证与脾胃气虚证有不同的结肠镜像 (P <0 .0 1)。肠粘膜苏木精 -伊红染色单个核细胞计数脾胃湿热组为 (32 .83± 7.0 1)个 /视野 ,明显高于脾胃气虚组 (17.71± 4 .79)个 /视野 (P <0 .0 1)及正常对照组 (14 .0 9± 5 .0 9)个 /视野 (P <0 .0 1) ,但后两组比较差异无显著性意义 (P >0 .0 5 )。脾胃湿热组、脾胃气虚组、正常对照组 P16表达阳性率分别为 17.6 5 %、4 1.94 %、6 3.6 4 % ;TGF- α则为 5 2 .94 %、2 5 .81%、9.0 9% ,P16和TGF- α表达存在显著的相关关系 (P <0 .0 1)。结论 :久泄脾胃湿热证有一定的炎症基础。
Objective:To investigate the relationship between persistent diarrhea with spleen stomach damp heat type and the following factors:colonoscopic image,inflammation cells,expression of P16 and TGF α. Methods:Thirty four cases of spleen stomach damp heat (SSDH) type,31 cases of spleen stomach qi deficiency (SSQD) type and 11 common people were selected and the inflammation cells in colon mucosa,the expression of P16 and TGF α were observed. Results:The difference of colonoscopie image between SSDH and SSQD group was obviously significant (P<0.01). The number of monocytes in HE staining colon mucosa of SSDH was 32.83±7.01 in every visual field, markedly higher than that of SSQD (17.71±4.79, P<0.01) and that of control group (14.09±5.09, P<0.01).No significant difference was found between the latter two groups. The positive rate of P16 expression in SSDH group, SSQV group and control group was 17.65%, 41.94% and 63.64% respectively. That of TGF α expression was 52.94%, 25.81% and 9.09% respectively. The positive rate of Pl6 expression was obviously correlated to that of TGF α (P<0.01). Conclusion: Inflammation is more severe in patients with persistent diarrhea with SSDH type. Different immune situations exist in SSDH type and SSQD type.
出处
《中国中西医结合消化杂志》
CAS
2002年第1期8-10,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
福建省医药卫生科研基金资助项目 (No.974 0)
关键词
腹泻
结肠炎
脾胃湿热
脾胃气虚
P16蛋白
转化生长因子-Α
diarrhea
colitis
spleen stomach damp heat syndrome
spleen stomach qi deficiency
P16
transforming growth factor alpha
