摘要
目的测定硝苯地平阿替洛尔缓释双层片中 2组分含量。方法采用ODS(Hypersil,5 μm,4 6mm× 2 0 0mm)为填充剂 ,甲醇 水 磷酸 ( 70∶30∶0 1 )流动相的高效液相色谱法。结果硝苯地平和阿替洛尔线性分别为 0 4 0～ 1 2 0 μg ,0 80～ 2 38μg;回收率分别为 99 8%(RSD =1 0 %) ,99 9%(RSD =0 5 %)。
Objective Selecting a method is to determine nifedipine and atenolol in their sustained release two layer tablets.Method A method was establised for the quantitative determination of nifedipine and atenolol in their sustained release two layer tablets by HPLC. With a 4 6 mm×200 mm column packing of 5 μm Spherisorb C 18 used as the stationary phase,a mixture of methanol,water and phosphoric acid(70∶30∶0 1) used as the mobile phase.Result The method had a good linearity and correlation coefficient:nifedipine was between 0 40～1 20 μg,atenolol was between 0 80～ 2 38 μg .The recoveries were 99 7% and 99 9%,RSD was 0 84% and 0 43% respectively.Conclusion The method can evaluate well and truly quality of this preparation.
出处
《沈阳药科大学学报》
CAS
CSCD
2002年第1期38-40,共3页
Journal of Shenyang Pharmaceutical University
