摘要
以沙田柚实生苗不同外植体为对象 ,进行了离体培养再生体系建立研究 ,结果表明 :在以 MT为基础附加不同浓度 BA的培养基上 ,下胚轴与子叶均可再生出不定芽 ,二者达最大不定芽分化率所需的 BA浓度不同 ,其中下胚轴为 BA3mg/ L,子叶为 BA4 m g/ L+NAA0 .5 m g/ L。添加 0 .5 mg/ L 的 KT可明显地提高不定芽发生率。下胚轴、子叶丛生芽继代所需的激素浓度分别是 :BA2 .0 m g/ L+IAA0 .5、BA3.0 m g/ L+IAA0 .2 5 m g/ L。组培苗所需的生根条件为 1/ 2 MS+IBA(0 .5～ 1.0 ) m g/ L 或 ABT2 .0 mg/ L。以卡那霉素作选择剂时 ,下胚轴与子叶的选择浓度分别为 70 mg/ L、5 0 mg/ L。
These experiments studied the regeneration of different Explants of seedling in C granis.Adventitious shoot,from hypocotyls and cotyledon,can be induced on MT medium with different concentration 6 BA and NAA.Optimum concentration of hormone for adventitious shoot formation is BA 3 mg/L in hypocotyls,BA 4 mg/L and NAA 0 5 mg/L in cotyledon.0 5 mg/L complement of KT in medium can enhance rate of adventitious shoot formation.Concentration of hormone in subculture is BA 2 0 mg/L+IAA 0 5 mg/L for hypocotyls,and BA 3 0 mg/L+IAA 0 25 mg/L for cotyledon.Ingredient of medium for rooting is 1/2 MS+IBA(0 5~1 0)mg/L or ABT 2 0 mg/L.As a selectable agent,concentration of Kanamycin is 70 mg/L for hypocotyls,50 mg/L for cotyledon.
出处
《广西林业科学》
2001年第4期166-168,共3页
Guangxi Forestry Science
