摘要
采用高效毛细管电泳电导检测法分离麻黄碱和伪麻黄碱 ,初步探讨了分离机理 ,建立了检测方法 .以柠檬酸 -柠檬酸钠为缓冲体系 ,铜盐为络合剂 ,在 p H值为 4 .5、电压 1 3.5 k V的条件下 ,盐酸麻黄碱和伪麻黄碱得到了较好的分离 ,加入适量乙醇可改善峰形和分离效果 .用该法以水杨酰胺为内标 ,对含盐酸麻黄碱和伪麻黄碱的实际样品进行检测 ,回收率为 97.3%～ 1 0 1 .1 % ,结果令人满意 .
The separation between ephedrine and pseudoephedrine with capillary electrophoresis and determination by the conductivity detector is reported. The separation mechanism is studied and the determination method is found. The effects of complex reagent concentration, concentration and pH of the running buffer, separation voltage and the organic solvent concentration are investigated and discussed. At 13.5 kV high voltage the ephedrine and pseudoephedrine are separated successfully in a citric acid sodium citrate buffer(pH=4.5) with the copper salt as the complex reagent. Ethanol is used to improve the peak shapes and separation effects. There are an excellent linearity between peak area and concentration of analytes in the concentration range of 1.4-165 mg/L for ephedrine and 0.825-165 mg/L for pseudophedrine with the detection limits( S/N =3) of 0 825 and 0 198 for ephedrine and pseudophedrine respectively. Relative standard deviations(RSD) of the ephedrine and psudoephedrine are 3 2%(ephedrine) and 2 8%(psudoephedrine) based on the determination of seven times. The ephedrine and pseudoephedrine in collunarium are detected and the satisfaction results are obtained.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第2期199-202,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :2 96 75 0 33)
广东省自然科学基金 (批准号 :0 0 12 37)资助
