摘要
本文应用X-射线能量色散技术对猪卵泡内卵母细胞,培养不同时间卵母细胞和体外受精卵进行了表面元素的定性和定量分析,结果表明,(1)在卵母细胞和受精卵的表面均含有Na、Mg、Al、Si、S、Cl、K、Ca和Fe元素;(2)A级卵母细胞随着体外培养,Ca的含量逐渐升高,而K的含量逐渐降低;(3)B、C级卵母细胞随着其培养Al、Ca^(2+)降低;(4)受精后卵表面的Ca显著升高。
With the X-ray energy chromatic dispersion technique.the surface elements of pig oocytes and external fertilized eggs were analysed qualitatively and quantitatively. The results showed as follows: (1 )The surface of the oocytes and fertilized eggs contained Na.Mg,Al,Si,S.Cl,K,Ca and Fe; (2)As the oocytes matured progress,their Ca content increased(P<0. 05) ;their K content decreased(P<0. 05); (3)After maturation theirr Al, Ca contents decreasde significantly(P<0. 01); (4)After fertilization ,the Ca content increased significant-ly(P<0. 01).
出处
《生物技术》
CAS
CSCD
1991年第3期12-15,共4页
Biotechnology
基金
国家农业部七五资助课题
关键词
猪
卵母细胞
体外受精
元素
pig, oocytes, external fertilization, elements
