摘要
为了评价急性心肌梗死患者氧化损伤程度及与抗氧化酶活性的关系。采用鲁米诺依赖的中性粒细胞化学发光法 ,对 6 0例急性心肌梗死患者及 6 2例年龄和性别匹配的健康对照者 ,检测外周血中性粒细胞产生氧自由基的水平 ;采用化学定量法测定血脂质过氧化终末产物———丙二醛的浓度及超氧化物歧化酶、谷胱甘肽过氧化物酶、肌酸磷酸激酶的活性。结果发现 ,急性心肌梗死组中性粒细胞化学发光峰值、积分、吞噬指数、血浆丙二醛浓度较对照组明显升高 (P均 <0 .0 0 1) ;血浆超氧化物歧化酶和谷胱甘肽过氧化物酶活性较对照组均明显降低 (P均 <0 .0 0 1) ;中性粒细胞化学发光峰值与血浆丙二醛浓度及血清肌酸磷酸激酶活性存在明显的正相关 ,前者r=0 .5 8(F =148.9,P <0 .0 1,n =12 2 ) ,相关方程为Y =- 482 4.9+144 1.2X ;后者r=0 .47(F =6 8.9,P <0 .0 1,n =12 2 ) ) ,相关方程为Y =1190 .8+0 .730 4X。中性粒细胞化学发光峰值与血浆超氧化物歧化酶活性呈显著的负相关 ,其相关系数为r=- 0 .6 3 (F =193 .2 ,P <0 .0 1,n =12 2 ) ,相关方程为Y =82 30 .0 - 78.1X ,。此结果提示 ,急性心肌梗死患者体内存在着明显的氧化损伤 ,同时也导致体内抗氧化能力减弱 ,氧化损伤的程度可能与心肌梗死的范围有关。
Aim We sought to evaluate the markers of polymorphonuclear (PMN) oxygen free radical (OFR) production, the levels of lipid peroxidation (malonaldialdehyde, MDA) and defenses against oxidative stress in the patients with acute myocardial infarction (AMI). Methods and Results A convenient and sensitive method for PMN OFR production was assayed by polymorphonuclear chemiluminescence (PMN-CL). We have measured the parameters of PMN-CL, the plasma MDA concentration, the plasma activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) in 60 AMI patients and 62 age-matched healthy subjects. Patients with AMI showed significantly increased PMN-CL parameters as peak value, integration and phagocytic index compared to healthy controls (P<0.001,respectively). The plasma MDA concentration increased in AMI patients compared with that in healthy controls (P<0.001). The PMN-CL peak value correlated best with plasma MDA concentration(r=0.58,P<0.01,n=122) and negatively correlated with the plasma activity of SOD (r=-0.63,P<0.01, n=122). Conclusion The PMN act as a potential contributor to extension of myocardial necrosis induced by OFR, thereby indicating the involvement of free radicals in AMI.
出处
《中国动脉硬化杂志》
CAS
CSCD
2001年第4期319-321,共3页
Chinese Journal of Arteriosclerosis
