摘要
目的 研究紫杉醇对小鼠巨噬细胞产生TNF α的影响。方法 按常规方法收集纯化 8～ 12周龄BALB/C雌性小鼠腹腔巨噬细胞 ,用RPMI16 4 0培养基培养 ,按所用药物紫杉醇不同浓度和 (或 )干扰素γ进行分组并设对照组。TNF α测定用ELISA试剂盒。结果 一定浓度的紫杉醇能够诱导巨噬细胞产生TNF α ,且随浓度的增加而增加 ;紫杉醇联合干扰素γ时 ,诱导作用明显增加。结论 紫杉醇的抗肿瘤作用与活化巨噬细胞 ,促进巨噬细胞产生细胞毒分子有关 。
Objective To study the effect of Paclitaxel on TNF α production of murine macrophage.Methods 8～12 week old BALB/c female mice were used.Peritonal macrophages were collected inRPMI 1640 culture.Peritonal macrophages were divided into groups according to the reagents(Paclitaxel and/or IFN γ).Peritonal macrophages without reagents were served as control group.TNF α was measured by TNF α specific enzymelinked immunosorbent assay(ELISA).Results Paclitaxel with certain concentration could induce macrophages to produc TNF α and the effect became strong with concentration increasing.Paclitaxel showed stronger effect on macrophages in combination with IFN-γ.Conclusions The antitumorigenic function of paclitaxel was related to activating murine peritoneal macrophages to promote the production of the cytotoxic molecules.
出处
《河南肿瘤学杂志》
2001年第5期322-323,共2页
Henan Journal of Oncology
关键词
紫杉醇
巨噬细胞
肿瘤坏死因子
paclitaxel
macrophage
TNF(tumor necrosis factor)
