摘要
目的 研究碱性成纤维细胞生长因子 (bFGF)对庆大霉素 (GE)所致豚鼠肾损伤的拮抗作用。方法 2 0只豚鼠随机分成三组 ,正常对照组 (N ,n =6) ,庆大霉素对照组 (G ,n =7) ,庆大霉素bFGF组 (F ,n =7)。G组 :GE 80mg·kg- 1 ·d- 1 肌肉注射 ,连续 1 5d ;F组 :bFGF肌肉注射 80 0U·kg- 1 ·d- 1 ,相隔 1～ 2h后加GE 80mg·kg- 1 ·d- 1 肌肉注射 ,连续 1 5d。第 1 6d取肾固定 ,石蜡包埋切片 ,HE染色 ,光镜观察肾小球、肾小管及肾间质。结果 F组与G组比较 ,F组肾小球毛细血管扩张、充血及内皮细胞肿胀较轻 ;肾小管坏死数量较少 ;肾小管再生、肾间质炎细胞浸润和纤维细胞增生明显。
Objective To study the antitoxic effect of basic fibroblast growth factor(bFGF) against guinea pig kidney injury induced by gentamicin. Method Gentamicin control group(G) was injected gentamicin 80mg·kg -1 ·d -1 im.for 15d;gentamicin & bFGF group(F) was injected with bFGF 800U·kg -1 ·d -1 im. and gentamicin 80mg·kg -1 ·d -1 im.for 15d. On the 16th day,the guinea pigs were killed and the kidney were detected by microscope. Result Compare with group G,capillary ectasia of renal corpuscle,hyperemia,endodermis cell swollen were lighter;necrosis of renal tubule was lesser;regeneration of renal tubule and fibre cell,infiltrate of inflammation cell were more distinctness in group F. Conclusion bFGF has protective effect on guinea pig kidney injury induced by gentamicin.
出处
《解剖学研究》
CAS
2001年第4期310-312,共3页
Anatomy Research
