摘要
小柴胡汤对大鼠肝线粒体单胺氧化酶B(MAO-B)有显著的抑制作用。IC^(50)为91.20mg/ml.单味药研究表明,组成小柴胡汤的7味中药显著地抑制MAO-B活性。按作用强弱依次为甘草>半夏>柴胡>党参>大枣>生姜>黄芩。动力学研究表明,小柴胡汤对MAO-B活性抑制作用属底物竞争性抑制。提示小柴胡汤对肝脏疾患及神经功能失调的治疗作用可能与其对MAO的抑制有关。
The inhibitory effect of Xiao chai hu Tang(XCHT) on the activity of mitochondrial monoamine oxidase B(MAO-B) of rat liver was observed. The concentration of XCHT exhibiting 50% inhibition on MAO-B activity in the reaction system was 91.20mg/ml. All the ingredient drugs which constitute XCHT showed significant inhibitory effect on MAO-B activity and their potencies were of the following order: Glycyrrhiza uralensis>Pinellia ternata>Bupleurum chinense>Codonopsis pilosula>Zinyphus jujuba var inermis>Zingiber officinale>Scutellaria baicalensis. Kinetic study showed that the inhibition of MAO-B by XCHT was substrate-competitive. The results suggest that the inhibition of MAO is one of the mechanisms of XCHT functioning in the treatment of some hepatic, neurological and psychiatric diseases in traditional medicine.
基金
沈阳药学院青年科学基金
关键词
小柴胡汤
单胺氧化酶B
Xiaochaihu Tang
Monoamine oxidase B
