摘要
用PAP和ABC法,观察了亮氨酸-脑啡肽(L-ENK)样免疫反应成分在52例胎龄为8-39周的人胎儿和2例新生儿脊髓的分布状况,并通过显微分光光度计对分布密度进行了量的变化的分析。L-ENK样阳性细胞在后角浅层始现于第16周胎儿,以后细胞数量随胎龄增长而逐渐增多,至第6个月达到高峰,此后却随胎龄增长而渐减,至降生前后已降至很少,即在胚胎发育过程中显示着“少—多—少”的变化规律。Ⅳ-Ⅶ层L-ENK样细胞出现略早(第14周),但数量很少且变化甚微,至第7个月以后,仅偶见散在的L-ENK样阳性细胞。L-ENK样阳性纤维和终末在后角浅层也始见于第16周胎儿,随胎龄增长而持续增多,至降生前后达到密和极密的程度。Ⅵ层内侧部(骶髓为后连合核)、Ⅶ层、网状核及其桕邻的侧索白质,L-ENK样纤维和终末从第14周开始出现,直至降生前后一直保持中等至密布的分布状态。
Using PAP and ABC methods and microspectrophotometry, the regularities of developmental changes and characteristics of the distribution of leucine-enkephalin-like immunoreactive (L-ENK-li) components in the spinal cord of the human foetuses (52 cases) and neonates (2 cases) have been examined.L-ENK-li cells were found in the superficial layers of the dorsal horn at the 16th week (foetus). As the foetuses grew, the immunopositive cells were progressively increased. At the 6th month, the number of L-ENK-li cells reached its maximum. However, they began to decrease gradually after the 7th month, and only a few cells were detectable in the neonates. The regularity of the above changes could be expressed as "low-high-low".The L-ENK-li cells in laminae Ⅳ, Ⅴ, Ⅵ and Ⅶ were present at the 14th week, and only a few could be observed. They were rarely found in these areas after the 7th month. The L-ENK-li fibers and terminals in the superficial layers of the dorsal horn showed a regular change, that is low to high, from the 16th week to the neonate. In the medial part of the lamina VJ (including the dorsal commissural nucleus of the sacral spinal cord), lamina Ⅶ and the reticular nucleus and the nearby white matter, these fibers maintained on a moderate or high level all along till birth.
出处
《神经解剖学杂志》
CSCD
北大核心
1991年第1期111-118,共8页
Chinese Journal of Neuroanatomy
关键词
亮氨酸
脑啡肽
免疫组织化学
脊髓
leucine-enkephalin
immunohistochemistry
spinal cord
human foetus and neonate
