摘要
目的 :探讨体外重建角膜组织的三维培养技术。方法 :原代培养角膜上皮、基质和内皮细胞 ,利用硫酸软骨素、透明质酸钠、 型胶原蛋白和微孔材料 ,建立三维培养系统 ,体外重建角膜组织结构 ,观察非醛交联、醛交联和微孔材料等三类人工角膜的透明度 ,常规病理组织学检测细胞在三维培养系统中生长状态。结果 :非醛交联类重建人工角膜质地较软 ,透明度较低 ,光镜可见纤维结构。醛交联类重建人工角膜质地较韧 ,透明性较高 ,光镜可见均质结构。非吸收微孔材料类细胞长入较少 ,较难体外重建人工角膜。结论 :利用生物工程技术能够体外重建人工角膜 ,非醛交联类结构较规则 。
Objective: To research the technology about reconstruction of cornea three-dimensional culture system in Vitra. Methods: using primary cultured corneal epithelial cells, stoma cells and endothelial cells rebuilt corneal structure in three dimensional culture system by hyaluronate, chondroitin, type I collagen and microporous materials. To exam transparency of nonaldehyde-derived cross-link, aldehyde-derived cross-link and microporous materials type artificial corneal by slit microcopy. The biological characters of artificial cornea are tested by routine pathological method. Results: The non-aldehyde-derived corss-link type artificial corneal is soften in character, lower in transparency and its fiber structure is visible under microscope. The aldehyde-derived cross-link type artificial corneal is tougher in character, higher in transparency and there is a homogeneous structure under microscope. Few of keratocytes can grow in micropore in microporous materials. It cannot re-built an artificial corneal by microporous materials. Conclusions: the transparent corneal structure can be rebuilt by tissue engineering technology in vita. The non-aldehyde-derived cross-link type artificial cornea has more regular structure and the aldehyde-derived cross-link type has more transparent than others.
出处
《中国实用眼科杂志》
CSCD
北大核心
2001年第11期815-817,T001-T002,共5页
Chinese Journal of Practical Ophthalmology
