摘要
目的 建立由动物自身产生的增生性瘢痕实验模型。方法 选用 4 7只日本大耳白兔 ,在耳腹侧面制作 6mm圆形全层皮肤缺损创面、1 5cm× 4 5cm长方形创面以及耳背圆形创面共3 88个 ,进行组织学等多项观察。结果 70 %兔耳圆形创面可发生与人增生性瘢痕类似的增生块 ,增生块持续时间最长为 15 0d ;长方形创面增生块发生率为 80 %以上 ,持续时间已超过 2 62d。增生块内有特征性的结节或漩涡状结构。创基注入TGF β1 、IFN γ ,可以促进或抑制增生块的发生。原位杂交 ,查明内源性TGF β1 mRNA为强阳性表达。利用此模型证实成纤维细胞凋亡在病理性瘢痕发生、发展过程中起着重要的调控作用。结论 兔耳可以产生与人增生性瘢痕类似的病理改变 ,可以用作瘢痕研究的实验模型。
Objective\ To estabish a real animal model for hypertrophic scar. Methods\ 388 wounds on the ears of 47 rabbits were created including rounds 6mm in diamiter on ventrol or dorsal side and 1 5cm×4 5cm rectangular wounds. Histological and histochemical analyses,in situ hybridization and cell apoptosis were tested. Results\ 70 percent of the wounds can from excess dermal scarring which is similar to human hypertrophic scar.The hight of excess dermal scarring is as 3～4 times high as oringinal ventol skin.The excess scarring can last 150 days at the most. while 80 percent appears on rectangular wounds and it lasts more than 262 days.Large amount of fibroblasts,nodular and spiral structures exist in excessive dermal scarring.Local injection of TGF-β 1 and IFN-r can promote and inhibit the formation of excessive dermal scarring respectively. SDS PAGE shows that type Ⅲ collagen content increased in excessive dermal scarring. In situ hybridization shows long lasting expression of type Ⅰ and Ⅲ precollagen mRNA in excessive dermal scarrint. TGF-β 1 mRNA also cope with that of precollagen. Fibroblast apoptosis in excessive dermal scarring of this model indicate that fibroblast apoptosis plays an important role in the process of occurrance, development of abnormal scar. Conclusion\ After wounding,rabbit ears can produce excessive dermal scarring which is similar to human hypertrophic scar, This can be used as an experimental model for the study of cicatrix.;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2001年第5期276-278,共3页
Chinese Journal of Plastic Surgery
关键词
增生性瘢痕
动物模型
原位杂交
细胞凋亡
Hypertrophic scar
Animal model
In situ hybrisization
Apoptosis
