摘要
目的 了解基质金属蛋白酶组织抑制因子TIMP 1和TIMP 2蛋白及mRNA在肝硬化组织中的表达和分布状态。方法 以抗TIMP 1和TIMP 2单克隆抗体及TIMP 1和TIMP 2cDNA探针为试剂 ,采用免疫组织化学法和原位杂交技术检测肝硬化组织中TIMP 1和TIMP 2蛋白和mRNA。结果 40例肝硬化患者肝组织中TIMP 1和TIMP 2蛋白以及mRNA表达的阳性率为10 0 % ,TIMP 1阳性的组织TIMP 2亦为阳性 ,且TIMP 1强度高于TIMP 2 ,而正常肝组织未见阳性表达。TIMP 1和TIMP 2蛋白及mRNA表达的阳性信号均位于肝细胞胞浆中 ,细胞核中无表达。结论 肝硬化患者的肝细胞中存在TIMP 1和TIMP 2蛋白及mRNA的表达 ,其表达强度与肝组织病理改变呈正相关 ,这可能是通过抑制基质金属蛋白酶 (MMPs)的活性 ,使得细胞外基质 (ECM )降解减少而引起肝硬化。
Objective To study the expression and distribution of TIMP 1 and TIMP 2 in liver tissue of cirrhosis patient and to investigate the roles and pathogenesis of TIMP 1 and TIMP 2 in liver cirrhosis. Methods TIMP 1 and TIMP 2 proteins and mRNA were detected with immunohistochemistry and in situ hybridization methods using monoclonal antibodies and cDNA probes. Results mRNA and proteins of TIMP 1 and TIMP 2 were detected in all the liver tissues from 40 liver cirrhosis patients, all in cytoplasm but not nucleus. TIMP 1 and TIMP 2 were found co exist in all samples, while TIMP 1 concentration was higher. Conclusions mRNA and protein of TIMP 1 and TIMP 2 are found in all the cirrhosis patient samples. Liver TIMP 1 and TIMP 2 concentrations increase with the progression of liver cirrhosis, decrease the degradation of extracellular matrix proteins, resulting in the initiation and the development of liver fibrosis and liver cirrhosis.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2001年第6期352-354,共3页
Chinese Journal of Infectious Diseases
基金
中国博士后科学基金资助项目 (中博基 [1999] 10 )
关键词
肝硬化
金属蛋白酶组织抑制因子
细胞外基质
免疫组织化学
原位杂交
Liver cirrhosis
Tissue inhibitors of metalloproteinases (TIMP 1 and TIMP 2)
Extracellular matrix
Immunohistochemistry
In situ hybridization
