摘要
目的 :研究儿童口腔内的变链菌基因型与奶瓶龋易感性的关系。方法 :取 2 0名奶瓶龋及 2 0名无龋儿童牙菌斑及唾液接种于TYCSB培养基计数并筛选MS ,采用AP -PCR法鉴定变链菌基因型。结果 :奶瓶龋组儿童唾液及菌斑变链菌计数高于无龋组 ;并且有两种以上基因型变链菌的比例也高于对照组 (14/ 2 0vs5 / 2 0 )。结论 :患有奶瓶龋的儿童不仅变链菌计数高 ,而且多数被两个以上基因型的MS感染。
AIM: To identify the relationship between the genotype of mutans streptococci and nursing-bottle dental caries in children. METHODS:20 children with nursing-bottle caries were chosen and 20 caries-free children as control. The plaque and saliva were cultivated in the TYCSB medium to isolates and counts the mutans streptococcai. Finally arbitrarily primed polymerase chain reaction(AP-PCR) was used to distinguish the genotype of the mutans streptococcai. RESULTS: The children with nursing-bottle caries had higher count of mutans streptococci than that of caries-free children. 14/20 of them with more than one genotype were detected. Only 5/20 of caries-free children harboured more than one genotype. CONCLUSION: Children with nursing-bottle caries were not only infected heavily with MS, but also often coloized with more than one clonal type.
出处
《牙体牙髓牙周病学杂志》
CAS
2001年第6期371-373,共3页
Chinese Journal of Conservative Dentistry
关键词
奶瓶龋
细菌学
变形链球菌
随机引物PCR
nursing-bottle caries
bacteriology
mutans streptococci genotype
AP-RCR
