摘要
目的 研究不同成分磷脂与巨噬细胞膜的结合特性及对LPS CD14结合的影响 ,阐明磷脂的抗炎机制。方法 用14 C PE或14 C PS检测磷脂与膜的结合特性 ,同时观察小鼠血浆对结合的影响 ;并用CD14抗体阻断法测定磷脂对LPS CD14结合的影响。结果 在正常小鼠血浆存在条件下 ,随磷脂浓度增加 ,磷脂与细胞膜的结合量也增加 ,而14 C PS和14 C PE相比较与细胞结合更多。不加血浆的实验组 ,磷脂与细胞膜的结合量明显减少。PE( 10 μmol/L)能显著抑制14 C PE( 1μmol/L)与细胞膜结合 ,PS( 10 μmol/L)能非常显著抑制14 C PS( 1μmol/L)与细胞膜结合。CD14抗体能显著抑制14 C PE及14 C PS与细胞膜的结合 ,而小鼠IgG并无此抑制作用。结论 不同磷脂与细胞膜的结合量不同 ,不同磷脂在细胞膜上有各自的特异性位点 ;PE、PS与LPS结合至mCD14是同一或邻近位置 。
Objective To study the characteristics of different phospholipids binding to macrophage membrane and its effects on LPS CD14 binding. Methods Different phospholipids binding to macrophage membrane were detected by 14 C PE or 14 C PS and effects of mouse plasma on binding were studied at the same time. The effect of phospholipids on LPS CD14 binding were deteted by anti CD14 monoclonal antibodies inhibition method. Results Phospholipids binding to membrane were increased in the presence of mouse plasma, but PS bound to membrane with apparently higher affinity than PE. In 10 fold molar excess, PE inhibited 14 C PE and 14 C PS and psinhibited binding to membrane obviously. PE and PS binding to membrane was inhibited by CD14 anti monoclonal antibody CD14 but not by mouse IgG. Conclusion PS, PE and LPS bind to the same or nearby regions of mCD14, thus it can modulate inflammation caused by LPS.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第11期1306-1308,共3页
Journal of Third Military Medical University
