摘要
目的 研究内毒素脂多糖 (Lipopolysaccharide ,LPS)刺激下巨噬细胞膜脂改变对CD14表达的影响。方法 LPS体外刺激巨噬细胞 ,用Westernblot及RT PCR检测随时间变化细胞膜CD14mRNA及蛋白表达的变化 ;同时观察膜脂微环境变化对CD14表达的影响。结果 LPS刺激巨噬细胞 1hCD14表达增加 ,5h达高峰 ,8h降至正常水平 ;而CD14mRNA水平 3h达高峰 ,5h降低。LPS刺激巨噬细胞 5h后细胞膜脂成分降解 ,膜脂流动性降低 ,CD14表达增加 ;用脂质体预处理后膜脂含量增加 ,膜脂流动性增加 ,CD14表达降低。结论 LPS体外刺激能上调巨噬细胞膜CD14表达 ,且此调节作用可能至少发生在转录水平 ;LPS刺激巨噬细胞使主要膜脂成分降解 ,膜流动性降低 。
Objective To investigate the effect of cellular membrane phospholipids on CD14 protein expression of macrophage stimulated by LPS. Methods Changes of CD14 protein expression and CD14 mRNA of macrophages stimulated by LPS in vitro were determined by Western blotting and RT PCR. Effects of membrane phospholipids on CD14 protein expression were also detected. Results After stimulation by LPS, CD14 expression increased at 1 h, reached the peak value at 5 h and decreased to the normal level at 8 h but CD14 mRNA reached the peak value at 3 h and decreased at 5 h. The levels of phospholipids and membrane fluidity decreased at 5 h but CD14 protein expression increased after LPS stimulation. After pretreatment with liposomes, membrane phospholipid microenvironment improved and CD14 protein expression decreased. Conclusion LPS can up regulate CD14 protein expression, which might be regulated at least at the transcriptional level of the CD14 gene. Changes of membrane phospholipid microenvironment may be an important reason for the up regulation of CD14 induced by LPS.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第17期1536-1539,共4页
Journal of Third Military Medical University
