摘要
目的 : 利用四氧嘧啶诱发大鼠体内广泛脂质过氧化 ,研究β-胡萝卜素 (βC)对红细胞脂质过氧化和红细胞膜流动性的影响。方法 : 选用 2 m龄 Wistar大鼠 44只 ,按性别、体重随机分为 A、B、C、D四组 ,其中 A、B两组分别喂予基础饲料 ,C、D两组分别喂含维生素 E(VE2 0 0mg/kg)和含天然 βC晶体 (2 0 0 mg/kg)的基础饲料。饲养 4w后 ,除 A组外 ,在空腹状态下腹腔注射四氧嘧啶 ,制成人工高自由基动物模型 ,48h后宰杀动物 ,收集血液 ,摘取肝脏 ,测红细胞膜、血清、肝匀浆中丙二醛 (MDA)含量 ,全血谷胱甘肽过氧化物酶 (GSH- Px)活性、红细胞膜 Na+ ,K+ -ATP酶活性及细胞膜流动性。结果 : 1 .四氧嘧啶可使 GSH- Px、Na+ ,K+ - ATP酶活性下降 (P<0 .0 1 ) ,MDA含量增高 ,红细胞膜流动性下降 (P<0 .0 1 )。 2 .添加βC或 VE后 ,GSH- Px、Na+ ,K+ -ATP酶活性、细胞膜流动性明显升高 (P<0 .0 5 ) ,MDA含量明显降低 (P<0 .0 5 )。结论 : βC或VE能降低体内脂质过氧化 ,从而保护红细胞膜结构与功能的稳定。
Objective: To study the effect of β- carotene(β-C) on lipid peroxidation and RBC-membrane fluidity of rats inflicted with alloxan. Methods: Forty-four 2mo-old Wistar rats were randomly divided into 4 groups and fed with basal diet (group A and B), basal diet + vitamin E (200 mg/kg) (C) and basal diet+β carotene (200 mg/kg) (D) for 4 w respectively. Then, alloxon (3%) was injected intraperitoneally except group A, which was injected with normal saline. After 48 h the rats were killed and a series of indices were examined. Results: After injected with alloxan, Na +,K +-ATPase and GSH-Px activity, RBC-membrane fluidity in rats fed with diets C,D were markedly increased while MDA levels were decreased. Conclusion: β-C and V E can decrease lipid peroxidation and increase the stability of RBC-membrane in rats inflicted with alloxan.
出处
《营养学报》
CAS
CSCD
北大核心
2001年第4期306-308,共3页
Acta Nutrimenta Sinica
